Somaclonal variation in watercress for resistance to crook root disease

Watercress (Rorippa nasturtium-aquaticum) shoots were regenerated from callus initiated from petioles on a range of media. The best coefficient of regeneration was obtained when callus was induced on a medium containing 0.25 mg l-1 thidiazuron and 0.05 mg l-1 2,4-D, followed by regeneration on 0.25...

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Bibliographic Details
Published in:Plant cell reports 1995, Vol.14 (4), p.241-244
Main Authors: Arnold, D.L, Flegmann, A, Clarkson, J.M
Format: Article
Language:English
Subjects:
2,4-D
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
callus
clones
cloning
culture media
culture techniques
disease resistance
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
in vitro culture
In vitro propagation: entire plant regeneration from tissues and cell cultures
Methods. Procedures. Technologies
Nasturtium officinale
pathogenicity
Pest resistance
plant breeding
Plant cells and fungal cells
plant pathogenic fungi
Plant pathogens
regenerative ability
screening
somaclonal variation
Spongospora subterranea
thidiazuron
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:Watercress (Rorippa nasturtium-aquaticum) shoots were regenerated from callus initiated from petioles on a range of media. The best coefficient of regeneration was obtained when callus was induced on a medium containing 0.25 mg l-1 thidiazuron and 0.05 mg l-1 2,4-D, followed by regeneration on 0.25 mg l-1 thidiazuron. Plants were then screened for resistance to crook root disease of watercress in a laboratory screening test which revealed significant somaclonal variation between the controls and some of the somaclones.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00233641