Poly(ethylene glycol)- and Carboxylate-Functionalized Gold Nanoparticles Using Polymer Linkages: Single-Step Synthesis, High Stability, and Plasmonic Detection of Proteins

Gold nanoparticles with suitable surface functionalities have been widely used as a versatile nanobioplatform. However, functionalized gold nanoparticles using thiol-terminated ligands have a tendency to aggregate, particularly in many enzymatic reaction buffers containing biological thiols, because...

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Bibliographic Details
Published in:Langmuir 2013-11, Vol.29 (44), p.13518-13526
Main Authors: Park, Garam, Seo, Daeha, Chung, Im Sik, Song, Hyunjoon
Format: Article
Language:English
Subjects:
Animals
Carboxylic Acids - chemistry
Cattle
Chemistry
Chemistry Techniques, Synthetic
Chymotrypsin - analysis
Chymotrypsin - chemistry
Colloidal state and disperse state
Exact sciences and technology
General and physical chemistry
Gold - chemistry
Metal Nanoparticles - chemistry
Methacrylates - chemistry
Nylons - chemistry
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Polyethylene Glycols - chemistry
Serum Albumin, Bovine - analysis
Serum Albumin, Bovine - chemistry
Surface Properties
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Summary:Gold nanoparticles with suitable surface functionalities have been widely used as a versatile nanobioplatform. However, functionalized gold nanoparticles using thiol-terminated ligands have a tendency to aggregate, particularly in many enzymatic reaction buffers containing biological thiols, because of ligand exchange reactions. In the present study, we developed a one-step synthesis of poly(ethylene glycol) (PEG)ylated gold nanoparticles using poly(dimethylaminoethyl methacrylate) (PDMAEMA) in PEG as a polyol solvent. Because of the chelate effect of polymeric functionalities on the gold surface, the resulting PEGylated gold nanoparticles (Au@P-PEG) are very stable under the extreme conditions at which the thiol-monolayer-protected gold nanoparticles are easily coagulated. Using the solvent mixture of PEG and ethylene glycol (EG) and subsequent hydrolysis, gold nanoparticles bearing mixed functionalities of PEG and carboxylate are generated. The resulting particles exhibit selective adsorption of positively charged chymotrypsin (ChT) without nonselective adsorption of bovine serum albumin (BSA). The present nanoparticle system has many advantages, including high stability, simple one-step synthesis, biocompatibility, and excellent binding specificity; thus, this system can be used as a versatile platform for potential bio-related applications, such as separation, sensing, imaging, and assays.
ISSN:0743-7463
1520-5827
DOI:10.1021/la402315a