Salidroside Inhibits Endogenous Hydrogen Peroxide Induced Cytotoxicity of Endothelial Cells

Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L., shows potent antioxidant property. Herein, we investigated the protective effects of salidroside against hydrogen peroxide (H2O2)-induced oxidative damage in human endothelial cells (EVC-304). EVC-304 cells were incubated in t...

Full description

Saved in:
Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2013/11/01, Vol.36(11), pp.1773-1778
Main Authors: Zhao, Xingyu, Jin, Lianhai, Shen, Nan, Xu, Bin, Zhang, Wei, Zhu, Hongli, Luo, Zhengli
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
apoptosis
Apoptosis - drug effects
Cell Line
Cell Survival - drug effects
Endothelial Cells - drug effects
Endothelial Cells - metabolism
endothelial injury model
Glucosides - pharmacology
Glutathione Peroxidase - metabolism
Humans
Hydrogen Peroxide
Malondialdehyde - metabolism
oxidative stress
Oxidative Stress - drug effects
Phenols - pharmacology
Proto-Oncogene Proteins c-bcl-2 - metabolism
salidroside
Superoxide Dismutase - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L., shows potent antioxidant property. Herein, we investigated the protective effects of salidroside against hydrogen peroxide (H2O2)-induced oxidative damage in human endothelial cells (EVC-304). EVC-304 cells were incubated in the presence or absence of low steady states of H2O2 (3–4 µ M ) generated by glucose oxidase (GOX) with or without salidroside. 3(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) assays were performed, together with Hoechst 33258 staining and flow cytometric analysis using Annexin-V and propidium iodide (PI) label. The results indicated that salidroside pretreatment attenuated endogenous H2O2 induced apoptotic cell death in EVC-304 cells in a dose-dependent pattern. Furthermore, Western blot data revealed that salidroside inhibited activation of caspase-3, 9 and cleavage of poly(ADP-ribose) polymerase (PARP) induced by endogenous H2O2. It also decreased the expression of Bax and rescued the balance of pro- and anti-apoptotic proteins. All these results demonstrated that salidroside may present a potential therapy for oxidative stress in cardiovascular and cerebrovascular diseases.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b13-00406