Construction of an RFLP map of barley

In order to construct an RFLP map of barley, two populations were analyzed using 251 genomic and cDNA markers: one population comprised 71 F1 antherderived double haploid (DH) individuals of an intraspecific cross (IGRI x FRANKA), and the other 135 individuals of an interspecific F2/F3 progeny (VADA...

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Bibliographic Details
Published in:Theoretical and applied genetics 1991-12, Vol.83 (2), p.250-256
Main Authors: Graner, A. (Federal Biological Research Center for Agriculture and Forestry, Gruenbach (Germany). Inst. for Resistance Genetics), Jahoor, A, Schondelmaier, J, Siedler, H, Pillen, K, Fischbeck, G, Wenzel, G, Herrmann, R.G
Format: Article
Language:English
Subjects:
Biological and medical sciences
CARTE GENETIQUE
Classical genetics, quantitative genetics, hybrids
Double haploids
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
GENETIC MAPS
GENETIC POLYMORPHISM
Genetics of eukaryotes. Biological and molecular evolution
HAPLOMETHODE
HAPLOMETHODS
HORDEUM VULGARE
Linkage map
MAPAS GENETICOS
METODO HAPLOIDE
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
RESTRICTION ENZYMES
Restriction fragment length polymorphism
Vegetals
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Summary:In order to construct an RFLP map of barley, two populations were analyzed using 251 genomic and cDNA markers: one population comprised 71 F1 antherderived double haploid (DH) individuals of an intraspecific cross (IGRI x FRANKA), and the other 135 individuals of an interspecific F2/F3 progeny (VADA x H. spontaneum). The distribution of nonrepetitive clones over the seven barley chromosomes revealed a maximum for chromosome 2H and a minimum for 6H. The polymorphism of the interspecific progeny (76%) clearly exceeded that of the intraspecific progeny (26%) although, based on their pedigrees, IGRI and FRANKA are only distantly related. The contribution of individual chromosomes of the DH parents to the overall polymorphism varied between 8% and 50%. A significant portion (44% versus 10% of the interspecific progeny) of the markers mapped on the DH offspring showed distorted segregation, caused mainly by the prevalence of variants originating from the parent that better responded to in vitro culture (IGRI). In contrast to the interspecific map, probes displaying skewed segregation were clustered on the DH map on discrete segments. The colinear arrangement of both maps covers a distance of 1,453 cM and identifies regions of varying map distances.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00226259