Physical maps of Nicotiana chloroplast DNA constructed by an efficient procedure

The restriction profiles of chloroplast DNA (cpDNA) from Nicotiana tabacum, N. sylvestris, N. plumbaginifolia, and N. otophora were obtained with respect to AvaI, BamHI, BglI, HindIII, PstI, PvuII, SalI, and XhoI. An efficient mapping method for the construction of cpDNA physical maps in Nicotiana w...

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Bibliographic Details
Published in:Theoretical and applied genetics 1992-02, Vol.83 (4), p.515-527
Main Authors: Yang, D.I. (National Tsing Hua Univ., Hsinchu (Taiwan). Inst. for Life Science), Feng, T.Y, Chen, C.C, Lai, Y.K
Format: Article
Language:English
Subjects:
APLICACIONES DEL ORDENADOR
APPLICATION DES ORDINATEURS
Biological and medical sciences
CARTE GENETIQUE
Chloroplast DNA
CHLOROPLASTE
CHLOROPLASTS
CLOROPLASTO
COMPUTER APPLICATIONS
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
Genes. Genome
GENETIC MAPS
MAPAS GENETICOS
Molecular and cellular biology
Molecular genetics
NICOTIANA
Physical mapping
RESTRICTION ENZYMES
Restriction patterns
Restriction site variations
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Summary:The restriction profiles of chloroplast DNA (cpDNA) from Nicotiana tabacum, N. sylvestris, N. plumbaginifolia, and N. otophora were obtained with respect to AvaI, BamHI, BglI, HindIII, PstI, PvuII, SalI, and XhoI. An efficient mapping method for the construction of cpDNA physical maps in Nicotiana was established via a computer-aided analysis of the complete cpDNA sequence of N. tabacum for probe selection. The efficiency of this approach is demonstrated by the determination of cpDNA maps from N. sylvestris, N. plumbaginifolia, and N. otophora with respect to all of the above restriction endonucleases. The size and basic structure of the cpDNA from the three species are almost identical, with an addition of approximately 80 bp in N. plumbaginifolia. The restriction patterns and hence the physical maps between N. tabacum and N. sylvestris cpDNA are identical and there is no difference in the Pvull digests of cpDNA from all four species. Restriction site variations in cpDNA from different species probably result from point mutations, which create or eliminate a particular cutting site, and they were observed spanning the whole chloroplast molecule but highly concentrated in both ends of the large, single-copy region. The results presented here will be used for the forthcoming characterization of chloroplast genomes in the interspecies somatic hybrids of Nicotiana, and will be of great value in completing the exploration of the phylogenetic relationships within this already extensively studied genus.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00226542