Serum cytokeratin 18 as a biomarker for gastric cancer

Cytokeratin 18 (CK18) fragments are released into circulation during epithelial cell death. M30 (reflects caspase-cleaved CK18 fragment) and M65 (reflects total CK18 fragment) enzyme-linked immunosorbent assay (ELISA) detect circulating CK18 fragments released during caspase-dependent or total cell...

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Bibliographic Details
Published in:Clinical and experimental medicine 2013-11, Vol.13 (4), p.289-295
Main Authors: Oyama, Katsunobu, Fushida, Sachio, Kinoshita, Jun, Okamoto, Koichi, Makino, Isamu, Nakamura, Keishi, Hayashi, Hironori, Inokuchi, Masafumi, Nakagawara, Hisatoshi, Tajima, Hidehiro, Fujita, Hideto, Takamura, Hiroyuki, Ninomiya, Itasu, Kitagawa, Hirohisa, Fujimura, Takashi, Ohta, Tetsuo
Format: Article
Language:English
Subjects:
Adult
Aged
Apoptosis
Biomarkers
Biomarkers - blood
Clinical Laboratory Techniques - methods
Cytokines
Enzyme-Linked Immunosorbent Assay - methods
Female
Gastric cancer
Hematology
Humans
Internal Medicine
Keratin-18 - blood
Male
Medical prognosis
Medicine
Medicine & Public Health
Middle Aged
Oncology
Original Article
Sensitivity and Specificity
Serum - chemistry
Stomach Neoplasms - diagnosis
Stomach Neoplasms - pathology
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Summary:Cytokeratin 18 (CK18) fragments are released into circulation during epithelial cell death. M30 (reflects caspase-cleaved CK18 fragment) and M65 (reflects total CK18 fragment) enzyme-linked immunosorbent assay (ELISA) detect circulating CK18 fragments released during caspase-dependent or total cell death, respectively; thus, CK18 has the potential of being a biomarker for epithelial cancers. In the present study, we investigated the serum levels of M30 and M65 in patients with gastric cancer, determined correlation of these levels with clinical features, and evaluated the usefulness of these enzymes as diagnostic and prognostic markers. We enrolled 54 gastric cancer patients and 12 healthy volunteers in this study. We measured the serum levels of M30 and M65 by quantitative ELISA. The levels of M30 and M65 in gastric cancer patients were significantly higher than those in healthy volunteers ( P  = 0.001, P  
ISSN:1591-8890
1591-9528
DOI:10.1007/s10238-012-0202-9