Plant regeneration from mesophyll protoplasts of Moricandia arvensis

Moricandia arvensis is of interest as it is a dicotyledonous species which has C3--C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the proto...

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Bibliographic Details
Published in:Plant cell reports 1992-07, Vol.11 (8), p.408-411
Main Authors: Murata, T, Mathias, R.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Brassicaceae
callus
culture media
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
laboratory techniques
mesophyll
Methods. Procedures. Technologies
Plant cells and fungal cells
Protoplast preparation, cell fusion and regeneration
protoplasts
regenerative ability
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Summary:Moricandia arvensis is of interest as it is a dicotyledonous species which has C3--C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the protoplasts which survived the first few days of culture produced calli. On a suitable regeneration medium, 30-60% of the calli regenerated one or more shoots. From among the regenerating shoots eight were selected, transferred to soil and grown to flowering in the glasshouse; all were fertile. The development of a protoplast regeneration system provides the opportunity to use transformation and somaclonal variation as tools in the genetic analysis of the C3--C4 character in this species.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00234371