Clonal multiplication of Gardenia jasminoides Ellis through axillary bud culture

• Published in: Plant cell reports 1993-11, Vol.13 (1), p.59-62
• Main Authors: GEORGE, P. S, RAVISHANKAR, G. A, VENKATARAMAN, L. V
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Plant cells and fungal cells
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/BF00232317

An efficient clonal multiplication system was developed for in vitro propagation of crocin - producing Gardenia jasminoides Ellis plants. Murashige and Skoog's (MS) medium containing 6-benzylaminopurine (BAP 1 mg l(-1)) and indole-3-butyric acid (IBA 1 mg l(-1)) resulted in multiple shoot initiation at the rate of 21 shoots per explant in 60 d of culture. Transfer of the microshoots into liquid MS medium supplemented with BAP (5 mg l(-1)) with two subcultures of 15 d duration in the same medium resulted in 400 ± 25 shoots per explant. Efficient rooting was achieved in MS medium supplemented with α-naphthaleneacetic acid (5 mg l(-1)). The in vitro raised plants were hardened in a greenhouse and transplanted to the field successfully. The method described will be useful for rapid multiplication of Gardenia for commercial exploitation.