A phosphoramidon-sensitive metalloprotease induces apoptosis of human endothelial cells by Group B Streptococcus

We explored Group B Streptococcus (GBS)-induced apoptosis in human umbilical vein endothelial cells (HUVEC) and the role of phosphoramidon, a zinc metalloprotease inhibitor, in this process. GBS 90186 strain (serotype V, a blood isolate) and concentrated supernatant (CS) were used to investigate the...

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Published in:Antonie van Leeuwenhoek 2013-12, Vol.104 (6), p.1125-1133
Main Authors: dos Santos, Michelle Hanthequeste Bittencourt, da Costa, Andréia Ferreira Eduardo, Ferreira, Beatriz Jandre, Souza, Simone Lima, da Silva Lannes, Pamella, Santos, Gabriela Silva, Mattos-Guaraldi, Ana Luiza, Nagao, Prescilla Emy
Format: Article
Language:English
Subjects:
Annexin A5 - analysis
Apoptosis
Biomedical and Life Sciences
Cell Survival
Cells, Cultured
Electrophoresis
Endothelial Cells - microbiology
Endothelial Cells - physiology
Glycopeptides - metabolism
Humans
Life Sciences
Medical Microbiology
Metalloproteases - antagonists & inhibitors
Metalloproteases - metabolism
Microbiology
Microscopy, Electron
Mortality
Neonates
Original Paper
Plant Sciences
Protease Inhibitors - metabolism
Proteases
Soil Science & Conservation
Staining and Labeling - methods
Streptococcus agalactiae - enzymology
Streptococcus infections
Trypan Blue - metabolism
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creator dos Santos, Michelle Hanthequeste Bittencourt
da Costa, Andréia Ferreira Eduardo
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Mattos-Guaraldi, Ana Luiza
Nagao, Prescilla Emy
description We explored Group B Streptococcus (GBS)-induced apoptosis in human umbilical vein endothelial cells (HUVEC) and the role of phosphoramidon, a zinc metalloprotease inhibitor, in this process. GBS 90186 strain (serotype V, a blood isolate) and concentrated supernatant (CS) were used to investigate the viability and morphological alterations in HUVEC by Trypan blue uptake, electrophoresis in 2 % agarose gel and scanning electron microscopy assays. Apoptosis before and after phosphoramidon-treatment were verified by flow cytometry using annexin V-FITC labeling. Differences were considered significant when P  
doi_str_mv 10.1007/s10482-013-0034-y
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subjects Annexin A5 - analysis
Apoptosis
Biomedical and Life Sciences
Cell Survival
Cells, Cultured
Electrophoresis
Endothelial Cells - microbiology
Endothelial Cells - physiology
Glycopeptides - metabolism
Humans
Life Sciences
Medical Microbiology
Metalloproteases - antagonists & inhibitors
Metalloproteases - metabolism
Microbiology
Microscopy, Electron
Mortality
Neonates
Original Paper
Plant Sciences
Protease Inhibitors - metabolism
Proteases
Soil Science & Conservation
Staining and Labeling - methods
Streptococcus agalactiae - enzymology
Streptococcus infections
Trypan Blue - metabolism
title A phosphoramidon-sensitive metalloprotease induces apoptosis of human endothelial cells by Group B Streptococcus
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