Chronic retene exposure causes sustained induction of CYP1A activity and protein in rainbow trout (Oncorhynchus mykiss)

The removal of persistent chlorinated organic compounds from pulp mill effluents has not eliminated mixed function oxygenase (MFO) induction by these effluents. Therefore, continuous MFO induction downstream of pulp mills may be due to exposure to more labile compounds, such as retene (an alkyl-subs...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 1998-11, Vol.17 (11), p.2347-2353
Main Authors: Fragoso, N.M. (Queen's University, Kingston, Ontario, Canada.), Parrott, J.L, Hahn, M.E, Hodson, P.V
Format: Article
Language:English
Subjects:
Agnatha. Pisces
AGUAS RESIDUALES
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
CITOCROMO P450
CYTOCHROME P450
Cytochrome P4501A
EAU USEE
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
ELABORACION DE PULPA
Ethoxyresorufin-O-deethylase
Fundamental and applied biological sciences. Psychology
Induction
ONCORHYNCHUS MYKISS
POLLUTION DE L'EAU
POLUCION DEL AGUA
Pulp mill effluent
PULPING
REDUCTION EN PATE
Retene
TOXICIDAD
TOXICITE
TOXICITY
WASTEWATER
WATER POLLUTION
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Summary:The removal of persistent chlorinated organic compounds from pulp mill effluents has not eliminated mixed function oxygenase (MFO) induction by these effluents. Therefore, continuous MFO induction downstream of pulp mills may be due to exposure to more labile compounds, such as retene (an alkyl-substituted phenanthrene), which typically cause transient induction after a single brief exposure. Because fish are exposed continuously to pulp mill effluents, we have tested, and rejected, the null hypothesis that continuous exposure of fish to retene does not cause sustained MFO induction. Rainbow trout exposed continuously to retene, a component of some pulp mill effluents and sediments downstream of pulp mills, showed concentration-dependent increases in hepatic ethoxyresorufin-O-deethylase (EROD) activity. The increase in EROD activity was sustained over 32 d of continuous exposure, but it diminished to background levels within 4 d after transfer to clean water. The enzymatic response was confirmed by measuring changes in the content of immunodetectable cytochrome P4501A (CYP1A) protein. These data support a role for labile, nonhalogenated compounds in chronic effects of pulp mill effluents on fish
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.5620171127