Metagenomic Analysis of Healthy and White Plague-Affected Mussismilia braziliensis Corals

Coral health is under threat throughout the world due to regional and global stressors. White plague disease (WP) is one of the most important threats affecting the major reef builder of the Abrolhos Bank in Brazil, the endemic coral Mussismilia braziliensis. We performed a metagenomic analysis of h...

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Bibliographic Details
Published in:Microbial ecology 2013-05, Vol.65 (4), p.1076-1086
Main Authors: Garcia, Gizele D., Gregoracci, Gustavo B., de O. Santos, Eidy, Meirelles, Pedro M., Silva, Genivaldo G. Z., Edwards, Rob, Sawabe, Tomoo, Gotoh, Kazuyoshi, Nakamura, Shota, Iida, Tetsuya, de Moura, Rodrigo L., Thompson, Fabiano L.
Format: Article
Language:English
Subjects:
Actinomycetales
Alveolata
Animals
Anthozoa
Anthozoa - microbiology
Anthozoa - parasitology
Apicomplexa
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Biological and medical sciences
Biomedical and Life Sciences
Brazil
classification
Coral reefs
Corals
Cyanobacteria
DNA
Ecology
Eukaryota
Eukaryota - classification
Eukaryota - genetics
Eukaryota - isolation & purification
Firmicutes
Fundamental and applied biological sciences. Psychology
genetics
Geoecology/Natural Processes
HOST MICROBE INTERACTIONS
isolation & purification
Life Sciences
Metagenomics
Microbial Ecology
Microbiology
microorganisms
Miozoa
Mussismilia braziliensis
Nature Conservation
Neisseriales
parasitology
plague
Proteobacteria
Reefs
Retrotransposons
Retroviridae
Rickettsiales
Vibrio
Vibrionales
Water Quality/Water Pollution
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Summary:Coral health is under threat throughout the world due to regional and global stressors. White plague disease (WP) is one of the most important threats affecting the major reef builder of the Abrolhos Bank in Brazil, the endemic coral Mussismilia braziliensis. We performed a metagenomic analysis of healthy and WP-affected M. braziliensis in order to determine the types of microbes associated with this coral species. We also optimized a protocol for DNA extraction from coral tissues. Our taxonomic analysis revealed Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, and Actinomycetes as the main groups in all healthy and WP-affected corals. Vibrionales, members of the Cytophaga—Flavobacterium—Bacteroides complex, Rickettsiales, and Neisseriales were more abundant in the WP-affected corals. Diseased corals also had more eukaryotic metagenomic sequences identified as Alveolata and Apicomplexa. Our results suggest that WP disease in M. braziliensis is caused by a polymicrobial consortium.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-012-0161-4