An Experimental Model of Nephritis Induced by Calf Serum Injection in Mice

• Published in: Japanese journal of pharmacology 1984, Vol.36(2), pp.223-234
• Main Authors: TSUCHIMOTO, Masahiro, KOMORIYA, Keiji, KOYAMA, Tamotsu, HOSODA, Kenji, TAKESHITA, Toru, NARUCHI, Tatsuyuki, MATSUURA, Eiji, YAMAMOTO, Itaru
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - therapeutic use; Biological and medical sciences; Blood Cell Count; Body Weight - drug effects; Cyclophosphamide - therapeutic use; Dexamethasone - therapeutic use; Disease Models, Animal; Fluorescent Antibody Technique; Glomerulonephritis; Immunochemistry; Indomethacin - therapeutic use; Kidney Glomerulus - pathology; Male; Medical sciences; Mercaptopurine - therapeutic use; Mice; Mice, Inbred ICR; Nephritis - drug therapy; Nephritis - etiology; Nephritis - immunology; Nephritis - pathology; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Organ Size - drug effects; Serum Sickness - complications; Serum Sickness - immunology
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1254/jjp.36.223

Acute glomerulonephritis characterized by proteinuria, hypoalbuminemia and leukocytosis was induced in mice by repeated intraperitoneal injections of calf serum (1 ml/mouse×10). In mice treated with calf serum, hypercellularity, karyorrhexis, expansion of the mesangium and hyalinosis in the glomeruli were observed by light microscopy. Furthermore, circulating immune complexes were detected in the serum, and deposits of mouse IgG and C3 on the basement membranes of the glomeruli were demonstrated immunohistochemically. Oral administration of cyclophosphamide or 6-mercaptopurine at a dose of 20 mg/kg/day significantly suppressed the development of this nephritis. Dexamethasone (0.5 mg/kg/day) caused moderate inhibition of the nephritic changes. These results suggest that this experimental model may be useful for evaluation of anti-nephritic drugs.