Effect of maintaining rainbow trout in creosote microcosms on lens optical properties and liver 7-ethoxyresorufin-O-Deethylase (EROD) activity

• Published in: Archives of environmental contamination and toxicology 2000-04, Vol.38 (3), p.350-356
• Main Authors: Whyte, J.J, Herbert, K.L, Karrow, N.A, Dixon, D.G, Sivak, J.G, Bols, N.C
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Contaminants; creosote; Creosote - toxicity; Cytochrome P-450 CYP1A1 - metabolism; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; environmental exposure; enzyme activity; eye lens; Fish; Fundamental and applied biological sciences. Psychology; Lens, Crystalline - pathology; Liver; Liver - drug effects; Liver - enzymology; Microcosms; Oncorhynchus mykiss; Oncorhynchus mykiss - physiology; Optical properties; pollutants; Polycyclic aromatic hydrocarbons; Salmon; toxicology; Trout; Water Pollutants, Chemical - toxicity; Water pollution
• ISSN: 0090-4341; 1432-0703
• DOI: 10.1007/s002449910046

Previously, exposure of fish to polycyclic aromatic hydrocarbons (PAHs) in both field and laboratory settings has been associated with eye damage, but this has only been expressed qualitatively. In this study, an automated scanning laser system has been employed to quantitatively evaluate changes in lens optical quality in rainbow trout (Oncorhynchus mykiss) following their in vivo exposure to creosote, which is a complex mixture with many PAHs. Rainbow trout were placed in 12,000-L outdoor microcosms dosed with 0, 3, or 10 microl/L liquid creosote for a 28-day period. Collected fish were examined for changes in focal length variability (FLV), lens size, and weight. These measurements were compared with induction of hepatic ethoxyresorufin-O-deethylase (EROD) activity and hepatic and water concentrations of priority pollutant PAHs. The optical quality of rainbow trout lenses was significantly reduced following creosote exposure, as indicated by increased FLV. Lens damage was significantly related to hepatic EROD activity, and both effects rose with creosote dose. Analytical measurements of microcosm water indicated elevated concentrations of PAHs in creosote-dosed ponds, including compounds capable of inducing rainbow trout EROD activity in vitro. Hepatic concentrations of PAHs were low and not related to creosote dose, likely due to rapid metabolism and elimination. This study demonstrates for the first time employment of a highly sensitive and quantitative technique to measure lens damage in fish exposed to contaminants in situ. The relationship between this effect and hepatic CYP1A activity may suggest a mechanistic linkage, which could lead to the use of EROD activity as an indicator of toxic effect rather than just chemical exposure.