Glucose-stimulated cAMP increase may be mediated by intracellular acidification in Saccharomyces cerevisiae

It has been reported that addition of glucose to cells of Saccharomyces cerevisiae grown on a sugar-free medium causes a peak of intracellular cAMP levels. Also, it has been proposed that this effect might be mediated by plasma membrane depolarization. However, here, we observed a hyperpolarizing ef...

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Bibliographic Details
Published in:FEBS letters 1985-07, Vol.186 (1), p.75-79
Main Authors: Caspani, Giorgio, Tortora, Paolo, Hanozet, Giorgio M., Guerritore, Andrea
Format: Article
Language:English
Subjects:
Biological and medical sciences
cAMP
FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone
Fundamental and applied biological sciences. Psychology
Glucose
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Intracellular acidification
Membrane potential
Mes, 4-morpholineethanesulfonic acid
Microbiology
Mycology
Saccharomyces cerevisiae
TPP+, tetraphenylphosphonium
Yeast
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Summary:It has been reported that addition of glucose to cells of Saccharomyces cerevisiae grown on a sugar-free medium causes a peak of intracellular cAMP levels. Also, it has been proposed that this effect might be mediated by plasma membrane depolarization. However, here, we observed a hyperpolarizing effect of glucose in S. cerevisiae and, in addition, no change in cAMP levels when depolarization was induced by valinomycin in the presence of K +. In contrast, treatments that induced a rapid intracellular acidification such as addition of the protonophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone at pH 5.5 but not at pH 8.0, extracellular pH shift from 8.5 to 3.5, and glucose itself, also increased the cyclic nucleotide. Thus, our data strongly support the hypothesis that intracellular acidification mediates the effect of glucose on cAMP levels.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)81342-9