Use of reflectance in soft laser densitometry in scanning opaque electrophoregrams: A high resolution technique

The present report describes a new approach in soft laser scanning densitometry performed on opaque electrophoregrams. The method employs a light reflectance system instead of absorption, which is commonly used. The data of this preliminary study were obtained by directly scanning a photograph of an...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1986-01, Vol.21 (1), p.25-30
Main Authors: Zeineh, R A, Kyriakidis, G
Format: Article
Language:English
Subjects:
densitometry
lasers
proteins
Online Access:Get full text
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Summary:The present report describes a new approach in soft laser scanning densitometry performed on opaque electrophoregrams. The method employs a light reflectance system instead of absorption, which is commonly used. The data of this preliminary study were obtained by directly scanning a photograph of an electrophoregram exhibiting viral proteins separated by SDS-PAGE. Comparison of the two methods clearly demonstrate that laser reflectance offers finer resolution than laser absorbance when opaque materials are scanned.
ISSN:0273-2289