Differentiation of anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components

Cuticular wax was extracted from adult females of Anopheles culicifacies Giles and analysed by gas liquid chromatography. The flies were taken from pure cage stocks of three sibling species: 56 individuals of species A, 43 of species B and 51 of species C. The three cytospecies were found to be sign...

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Bibliographic Details
Published in:Bulletin of entomological research 1986-09, Vol.76 (3), p.529-537
Main Authors: Milligan, P. J. M., Phillips, A., Molyneux, D. H., Subbarao, S. K., White, G. B.
Format: Article
Language:English
Subjects:
Anopheles culicifacies
Biological and medical sciences
Culicidae
cuticle
cytogenetics
disease vectors
Fundamental and applied biological sciences. Psychology
Insecta
Invertebrates
malaria
Original Articles
Pathology
Systematics. Geographical distribution
taxonomy
Tropical medicine
waxes
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Summary:Cuticular wax was extracted from adult females of Anopheles culicifacies Giles and analysed by gas liquid chromatography. The flies were taken from pure cage stocks of three sibling species: 56 individuals of species A, 43 of species B and 51 of species C. The three cytospecies were found to be significantly different in their cuticular hydrocarbon composition by a multivariate analysis of variance. Using discriminant analysis, each cytospecies was characterized by the amounts of C25-C33 hydrocarbons extracted from the cuticle, allowing criteria to be established for the identification of flies. By these criteria, specimens from stocks of known identity were allocated to the correct group with a high success rate. These results may reflect ecological differences among the sibling species.
ISSN:0007-4853
1475-2670
DOI:10.1017/S0007485300015017