Accumulation Forms of Zn and Pb in Phaseolus vulgaris in the Presence and Absence of EDTA

• Published in: Environmental science & technology 2001-07, Vol.35 (13), p.2854-2859
• Main Authors: Sarret, Géraldine, Vangronsveld, Jaco, Manceau, Alain, Musso, Martine, D'Haen, Jan, Menthonnex, Jean-Jacques, Hazemann, Jean-Louis
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Chelating Agents - chemistry; Economic plant physiology; Edetic Acid - chemistry; Fundamental and applied biological sciences. Psychology; Lead - pharmacokinetics; Mineral nutrition; Nutrition. Photosynthesis. Respiration. Metabolism; Phaseolus - chemistry; Plant Leaves - chemistry; Plant physiology and development; Plant Roots - chemistry; Pollutants; Soil Pollutants - pharmacokinetics; Soils; Solubility; Tissue Distribution; Trace elements; Water and solutes. Absorption, translocation and permeability; Zinc - pharmacokinetics
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es000219d

The internalized speciation of Zn and Pb in roots and leaves of Phaseolus vulgaris grown in zinc sulfate, zinc EDTA, lead nitrate, and lead EDTA solutions were studied by electron microscopy (Zn) and extended X-ray absorption fine structure (EXAFS) spectroscopy (Zn and Pb). Zn was predominantly present as Zn phosphate dihydrate in the roots and leaves of the plant regardless of its form in solution. Pb was predominantly found in the leaves as cerussite (lead carbonate) when the plant was grown in Pb nitrate solution and as a mixture of PbEDTA and an undetermined species in contact with PbEDTA solution. Therefore, Phaseolus vulgaris is able to dissociate totally (Zn) or partly (Pb) the two metal−EDTA complexes from the nutrient solution and to bind these metals in other forms.