Nucleotide sequence analysis of a cloned DNA fragment from human cells reveals homology to retrotransponsons

During molecular cloning of proviral DNA of human spumaretrovirus, various recombinant clones were established and analyzed. Blot hybridization revealed that one of the recombinant plasmids had the characteristic features of a member of the long interspersed repetitive sequences family. The DNA elem...

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Bibliographic Details
Published in:Molecular and cellular biology 1987-01, Vol.7 (1), p.231-236
Main Authors: Fluegel, R M, Maurer, B, Bannert, H, Rethwilm, A, Schnitzler, P, Darai, G
Format: Article
Language:English
Subjects:
amino acid sequence
DNA
embryo fibroblasts
man
mice
nucleotide sequence
Primates
proviruses
restriction endonuclease mapping
spumaretrovirus (human)
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Summary:During molecular cloning of proviral DNA of human spumaretrovirus, various recombinant clones were established and analyzed. Blot hybridization revealed that one of the recombinant plasmids had the characteristic features of a member of the long interspersed repetitive sequences family. The DNA element was analyzed by restriction mapping and nucleotide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when compared with the 5'-terminal part of the pol gene product of the murine retrotransposon LIMd. The 3' region of the cloned DNA element encodes proteins with an even higher degree of homology of 67.4% in comparison to the corresponding parts of a member of the primate KpnI sequence family.
ISSN:0270-7306