Plant regeneration from callus and protoplast cultures of Helianthus giganteus L

Methods of plant regeneration from callus and protoplasts of Helianthus giganteus L. are described. Embryogenic callus was obtained from leaf explants and plants were regenerated from these calli on MS media with different combinations of benzyladenine and naphtaleneacetic acid. Leaf protoplasts iso...

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Bibliographic Details
Published in:Plant cell reports 1992-02, Vol.11 (1), p.7-10
Main Authors: KRASNYANSKI, S, POLGAR, Z, NEMETH, G, MENCZEL, L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Plant cells and fungal cells
Protoplast preparation, cell fusion and regeneration
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Summary:Methods of plant regeneration from callus and protoplasts of Helianthus giganteus L. are described. Embryogenic callus was obtained from leaf explants and plants were regenerated from these calli on MS media with different combinations of benzyladenine and naphtaleneacetic acid. Leaf protoplasts isolated from in vitro grown plants formed somatic embryos when cultured in agarose solidified droplets of V-KM medium containing benzyladenine and naphtaleneacetic acid. Embryos developed into plantlets on media with reduced auxin contents. Regenerated plants were successfully planted in soil.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00231830