Agrobacterium-mediated transformation of pepino and regeneration of transgenic plants

Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avir...

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Bibliographic Details
Published in:Plant cell reports 1991-07, Vol.10 (4), p.208-212
Main Authors: ATKINSON, R. G, GARDNER, R. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Plant cells and fungal cells
Protoplast preparation, cell fusion and regeneration
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Summary:Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avirulent Agrobacterium tumefaciens strain LBA4404. Optimization of transformation protocols was rapidly achieved by monitoring early expression of the GUS (β-D-glucuronidase) reporter gene carried on pKIWI110. Transgenic plants expressed GUS and selectable marker genes for kanamycin resistance and chlorsulfuron resistance. PCR (polymerase chain reaction) and Southern analysis provided molecular evidence for transformation.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00234297