Production of aposporous gametophytes and calli from Pteris vittata L. Pinnae strips cultured in vitro

Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from p...

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Bibliographic Details
Published in:Plant cell reports 1991-10, Vol.10 (8), p.392-393
Main Authors: Kwa, S.H, Wee, Y.C, Loh, C.S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
callus
culture media
embryo (plant)
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
germ cells
growth
in vitro culture
In vitro propagation: entire plant regeneration from tissues and cell cultures
Methods. Procedures. Technologies
Plant cells and fungal cells
Pteris
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Summary:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata cultured in vitro at lower concentrations of all the mentioned components. Aposporous gametophytes and vegetative calli were produced at higher concentrations. The calli regenerated sporophytes when cultured on MS medium without growth regulators. The gametophytes grew vegetatively on MS medium but produced sporophytes when transferred into 0.1 strength MS medium. This is the first report of simultaneous production of calli and gametophytes from fern explants.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00232608