improbability of irradiated spermatozoa as gene transfer vectors in chickens

It is concluded that chromatin fragments derived from irradiated chicken spermatozoa are not viable vectors for gene transfer. In three experiments conducted at sequential intervals over a period of 1 1/2 years, no marker traits were found in 1,065 G0 progeny from irradiated spermatozoa of Minnesota...

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Bibliographic Details
Published in:Theoretical and applied genetics 1990-08, Vol.80 (2), p.228-233
Main Authors: Shoffner, R.N, Otis, J.S, Snyder, L.A, Guise, K.S
Format: Article
Language:English
Subjects:
artificial insemination
Biological and medical sciences
Biological effects of radiation
chickens
chromatin
chromatin fragments
dominance
fertilization (reproduction)
Fundamental and applied biological sciences. Psychology
gene frequency
gene transfer
genetic markers
genotype
Ionizing radiations
irradiation
spermatozoa
Tissues, organs and organisms biophysics
vectors
zygote
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Summary:It is concluded that chromatin fragments derived from irradiated chicken spermatozoa are not viable vectors for gene transfer. In three experiments conducted at sequential intervals over a period of 1 1/2 years, no marker traits were found in 1,065 G0 progeny from irradiated spermatozoa of Minnesota Dominant Marker males inseminated into recessive Rhode Island Red and White Leghorn females. The inability to secure transformants is ascribed to the following factors: a maximum of five and probably fewer potential vector fragments for each G0 progeny because of irradiation effect on spermatozoan ability to enter the germinal disc; uncertainty of DNA integrity from highly irradiated chromatin; no known mechanism for release of chromatin fragments from irradiated spermatozoa supernumerary pronuclei; and the uncertainty of selective integration into the zygotic nucleus.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00224391