Effects of ectopic HER-2/neu gene expression on the COX-2/PGE sub(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells

• Published in: Journal of experimental & clinical cancer research 2013-01, Vol.32 (1), p.11-11
• Main Authors: Li, Shu, Ma, XiaoXin, Ma, Li, Wang, Cuicui, He, YuanQi, Yu, ZhiJuan
• Format: Article
• Language: English
• ISSN: 1756-9966
• DOI: 10.1186/1756-9966-32-11

Objectives: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma. Methods: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA. Results: Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells. Conclusion: HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal.