Fish oil, melatonin and vitamin E attenuates midbrain cyclooxygenase-2 activity and oxidative stress after the administration of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine

• Published in: Metabolic brain disease 2013-12, Vol.28 (4), p.705-709
• Main Authors: Ortiz, Genaro Gabriel, Pacheco-Moisés, Fermín P., Gómez-Rodríguez, Víctor M., González-Renovato, Erika D., Torres-Sánchez, Erandhis D., Ramírez-Anguiano, Ana C.
• Format: Article
• Language: English
• Subjects: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cyclooxygenase 2 - metabolism; Fish Oils - pharmacology; Lipid Peroxidation - drug effects; Melatonin - pharmacology; Mesencephalon - drug effects; Mesencephalon - enzymology; Metabolic Diseases; Mice; Mice, Inbred C57BL; Neurology; Neurosciences; Oncology; Original Paper; Oxidative Stress - drug effects; Vitamin E - pharmacology
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-013-9416-0

Parkinson’s disease is a neurodegenerative disease whose hallmark pathological features include a selective loss of dopaminergic neurons in the midbrain. Ciclooxygenase-2 activity induction and oxidative stress have been implicated in the aetiology of Parkinson’s disease and in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) animal model of Parkinson disease. Upon administration of fish oil, melatonin and vitamin E, neuroprotective effects on MPTP-induced neurotoxicity have been indicated. The aim of this study was to investigate the time course and compare the potency of these agents alone, on several parameters such as COX-2 and lipid peroxides (LPO) products associated with MPTP neurotoxicity in midbrain homogenates of C57BL/6 mice. Using fish oil (0.0368 g EPA and 0.0184 g DHA, per day), melatonin (10 mg/kg/day), and vitamin E (50 mg/Kg/day) we have now shown that COX-2 activity, LPO and nitrite/nitrate levels were significantly increased in MPTP treated mice ( p