Gene therapy of gastric cancer using LIGHT-secreting human umbilical cord blood-derived mesenchymal stem cells

Background Mesenchymal stem cells (MSCs) have the ability to migrate into tumors and therefore are potential vehicles for the therapy of malignant diseases. In this study, we investigated the use of umbilical cord blood mesenchymal stem cells (UCB-MSCs) as carriers for a constant source of transgeni...

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Bibliographic Details
Published in:Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2013-04, Vol.16 (2), p.155-166
Main Authors: Zhu, Xinhong, Su, Dongming, Xuan, Shiying, Ma, Guiliang, Dai, Zhenbo, Liu, Tongyun, Tang, Dongqi, Mao, Weizheng, Dong, Chenfang
Format: Article
Language:English
Subjects:
Abdominal Surgery
Animals
Base Sequence
Cancer Research
Disease Models, Animal
Fetal Blood - cytology
Gastric cancer
Gastroenterology
Gene Expression Regulation
Genetic Therapy - methods
Humans
Lentivirus
Lentivirus - genetics
Male
Medicine
Medicine & Public Health
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal Stromal Cells - secretion
Mesenchymal Stromal Cells - virology
Mice
Mice, Nude
Molecular Sequence Data
Oncology
Original Article
Stomach Neoplasms - pathology
Stomach Neoplasms - therapy
Surgical Oncology
Tumor Necrosis Factor Ligand Superfamily Member 14 - genetics
Tumor Necrosis Factor Ligand Superfamily Member 14 - pharmacology
Tumor Necrosis Factor Ligand Superfamily Member 14 - secretion
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Summary:Background Mesenchymal stem cells (MSCs) have the ability to migrate into tumors and therefore are potential vehicles for the therapy of malignant diseases. In this study, we investigated the use of umbilical cord blood mesenchymal stem cells (UCB-MSCs) as carriers for a constant source of transgenic LIGHT (TNFSF14) to target tumor cells in vivo. Methods Lentiviral vectors carrying LIGHT genes were constructed, producing viral particles with a titer of 2 × 10 8  TU/L. Fourteen days after UCB-MSCs transfected by LIGHT gene packaged lentivirus had been injected into mouse gastric cancer models, the expression levels of LIGHT mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Then the tumors’ approximate volumes were measured. Results The treatment with MSC-LIGHT demonstrated a strong suppressive effect on tumor growth compared to treatment with MSC and NaCl ( p  
ISSN:1436-3291
1436-3305
DOI:10.1007/s10120-012-0166-1