Susceptibility of Field Populations of the Fall Armyworm (Lepidoptera: Noctuidae) from Florida and Puerto Rico to Purified Cry1f Protein and Corn Leaf Tissue Containing Single and Pyramided Bt Genes

• Published in: The Florida entomologist 2013-09, Vol.96 (3), p.701-713
• Main Authors: Niu, Ying, Meagher, Robert L, Yang, Fei, Huang, Fangneng
• Format: Article
• Language: English
• Subjects: Army-worms; Armyworms; Bacillus thuringiensis; Bioassay; bioassays; Bt susceptibility; Corn; corn protein; Cross resistance; Cry1F; Diet; Diseases and pests; Distribution; Environmental aspects; essential genes; Fall Armyworm Symposium Papers; genes; genes múltiples; Health aspects; Hybridity; hybrids; Insect larvae; Insect-plant relationships; larvae; leaves; Lepidoptera; lethal concentration 50; manejo de resistencia; maíz Bt; Mortality; multiple genes; Noctuidae; population; Population distributions; proteins; resistance management; resistance management; Bt corn; Spodoptera frugiperda; susceptibilidad Bt; transgenic plants
• ISSN: 0015-4040; 1938-5102
• DOI: 10.1653/024.096.0302

Larval survival of Cry1F-susceptible (FL), -resistant (PR and Cry1F-RR), and -heterozygous (FL x PR and Cry1F-RS) populations of the fall armyworm, Spodoptera frugiperda (J. E. Smith) to purified Cry1F protein and corn leaf tissue of 7 Bacillus thuringiensis (Bt) corn hybrids and 5 non-Bt corn hybrids was evaluated in the laboratory. The 7 Bt corn hybrids represent 5 Bt corn traits: Herculex®I, which expresses a single Bt protein (Cry1F), and Genuity® VT Double Pro™, VT Triple Pro™, SmartStax™, and Agrisure® Viptera® 3111, which contain ≥ 2 pyramided Bt genes. The original FL and PR populations were collected from corn fields in 2011 in Florida and Puerto Rico, respectively. Diet-incorporation bioassays showed that FL was susceptible to Cry1F protein with a LC50 value of 0.13–0.23 µg/g, while PR was highly resistant to Cry1F protein (> 137-fold). FL was also susceptible to all 7 Bt corn hybrids with a 7-day mortality of > 95%, while PR and a backcrossed and reselected population, Cry1F-RR, were highly resistant to Cry1F corn leaf tissue. The resistance was recessive or incompletely recessive. None of the 5 populations of S. frugiperda could survive on Viptera™ 3111, suggesting this Bt corn trait can completely overcome the resistance and thus should provide a means of managing Cry1F resistance in S. frugiperda. However, Cry1F-RR exhibited a significant cross-resistance to the leaf tissue of the other 3 pyramided Bt corn traits. The possible cross-resistance between single-gene and pyramided Bt corn products suggest that careful selection of Bt genes is essential in use of gene pyramiding strategy for resistance management.