Fast real‐time detection of Potato spindle tuber viroid by RT‐LAMP

This paper reports the development of a single tube, real‐time, reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) assay for detecting Potato spindle tuber viroid (PSTVd), one of the quarantine pathogens of potato in Europe and North America. The method enables detection of a bro...

Full description

Saved in:
Bibliographic Details
Published in:Plant pathology 2013-10, Vol.62 (5), p.1147-1156
Main Authors: Lenarčič, R., Morisset, D., Mehle, N., Ravnikar, M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Isothermal amplification
melting curve
Phytopathology. Animal pests. Plant and forest protection
potato
Potato spindle tuber viroid
PSTVd
RT‐LAMP
Solanum tuberosum
viroid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This paper reports the development of a single tube, real‐time, reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) assay for detecting Potato spindle tuber viroid (PSTVd), one of the quarantine pathogens of potato in Europe and North America. The method enables detection of a broad range of PSTVd isolates, and is about 10 times more sensitive than the conventional reverse transcription polymerase chain reaction (RT‐PCR) assay. Its benefits are not only its speed (15–25 min to obtain results) and cost effectiveness (resulting from time saved as well as cheaper consumables), but also its demonstrated ability to be performed in the field, using portable instruments.
ISSN:0032-0862
1365-3059
DOI:10.1111/ppa.12017