Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness

• Published in: Biology and fertility of soils 2013-11, Vol.49 (8), p.1053-1063
• Main Authors: Lopez, Blanca R., Bashan, Yoav, Trejo, Adan, de-Bashan, Luz E.
• Format: Article
• Language: English
• Subjects: Agriculture; Agronomy. Soil science and plant productions; Azospirillum brasilense; Bacteria; Biochemistry and biology; Biological and medical sciences; Biomedical and Life Sciences; Chemical, physicochemical, biochemical and biological properties; Chlorella sorokiniana; Community ecology; Community structure; Desert soils; Deserts; Detritus; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Life Sciences; Microalgae; Microbiology; Microorganisms; Original Paper; Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Plant growth; Population structure; Rhizosphere; Soil conditioners; Soil fertility; Soil microorganisms; Soil science; Soil Science & Conservation; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Soils; Species diversity; Species richness; Tertiary wastewater treatment
• ISSN: 0178-2762; 1432-0789
• DOI: 10.1007/s00374-013-0799-1

The main goal of this study was to expand our knowledge of what happens to the soil bacterial community in an eroded desert soil when improvement of soil fertility is derived from the application of debris of tertiary wastewater treatment containing immobilized microalgae Chlorella sorokiniana and the plant growth-promoting bacterium (PGPB) Azospirillum brasilense . We hypothesized that an “improved” non-agricultural desert soil will exhibit substantial changes in the structure of the bacterial community in a relatively short time after amendment. To assess the effect of the amendments, microalgae and PGPB alone or combined, on the structure of the rhizosphere bacterial community, changes in species richness and bacterial diversity over time were based on sequence differences in the 16S rRNA gene, performed with PCR–denaturing gradient gel electrophoresis (DGGE) and then analyzed by similarity test and non-metric multidimensional scaling analysis. Root surface colonization and persistence in the rhizosphere of A . brasilense was monitored by fluorescent in situ hybridization and sequencing of DGGE bands. Application of waste debris significantly changed the rhizosphere bacterial population structure, whether comparisons were made over time, between inoculated and non-inoculated soil, and among different inoculated microorganisms. Species richness and diversity increased when the waste debris contained the microalgae–bacteria association and also over time. Even as its secondary role as an inoculant after wastewater treatment, A . brasilense colonized the root surface profusely and persisted within the rhizosphere bacterial community. This study demonstrated that small organic amendment to desert soil significantly changed soil bacterial community compared to the original soil and also 2 months after amendments were added.