Responses of the cytochrome P-450 monooxygenase system to diesel oil in the common mussel, Mytilus edulis L., and the periwinkle, Littorina littorea L

• Published in: Aquatic toxicology 1985-01, Vol.7 (1), p.79-91
• Main Authors: Livingstone, David R., Moore, Michael N., Lowe, David M., Nasci, Cristina, Farrar, Suzanne V.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; cytochrome b5; cytochrome P-450; Fundamental and applied biological sciences. Psychology; Invertebrates; Littorina littorea; Marine; Mollusca; monitoring; mussel; Mytilus edulis; NADPH-cytochrome c reductase; NADPH-neotetrazolium reductase; periwinkle; Physiology. Development; pollution
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/0166-445X(85)90037-2

Mussels and periwinkles were exposed long term (4–16 mth) to 29 and 123 ppb diesel oil under natural environmental conditions. Mussels were also exposed for 8 days to 29 ppb, and long-term 29 ppb mussels were recovered for 8 days in clean water. Cytochromes P-450 and b 5 and NADPH-neotetrazolium and NADPH-cytochrome c reductase activities were elevated in digestive gland microsomes in long-term exposed molluscs, and the enzyme activities were highest in 123 ppb animals. Cytochrome P-450 was elevated in short-term exposed mussels, and returned to control concentrations in long-term exposed mussels following a recovery period of 8 days. Increases and decreases in P-450 were accompanied by, respectively, a 2–3 nm blue and red shift in the wavelength maximum of the P-450 peak. No increases were observed in benzo[ a]pyrene hydroxylase activity.