Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides

One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications. Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 1994-11, Vol.5 (11), p.955-958
Main Authors: Vorm, Ole, Mann, Matthias
Format: Article
Language:English
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Summary:One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications. Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used. A new procedure for preparing matrix surfaces is used, and there is no need to mix the matrix with the sample or to add internal standards. Examples are shown with a mass accuracy better than 50 ppm in a peptide mixture. Peptide mapping as well as sequencing by creating “ragged ends” or “ladder sequencing” should benefit especially from the improved mass accuracy.
ISSN:1044-0305
1879-1123
DOI:10.1016/1044-0305(94)80013-8