Long term culture and caffeine production of immobilized coffee (Coffea arabia) L. cells in polyurethane foam

Coffee (Coffea arabica L.) cells could be immobilized in polyurethane foam and subcultured repeatedly for a long time. Four phases were observed for cell growth and caffeine production, I; immobilization, II; growth, III; caffeine production, IV; regrowth. Their periods were influenced by the number...

Full description

Saved in:
Bibliographic Details
Published in:Plant cell reports 1990-07, Vol.9 (3), p.125-128
Main Authors: Furuya, T, Koge, K, Orihara, Y
Format: Article
Language:English
Subjects:
Biological and medical sciences
biosynthesis
Biotechnology
caffeine
cell culture
cell growth
Coffea arabica
Establishment of new cell lines, improvement of cultural methods, mass culture
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
immobilization
long term experiments
Methods. Procedures. Technologies
Plant cells and fungal cells
regrowth
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Coffee (Coffea arabica L.) cells could be immobilized in polyurethane foam and subcultured repeatedly for a long time. Four phases were observed for cell growth and caffeine production, I; immobilization, II; growth, III; caffeine production, IV; regrowth. Their periods were influenced by the number of foam particles. Especially in the phase III, the immobilized cells produced a relatively large amount of caffeine in the subculture numbers 5-8 (34 cubes) when the fresh weight of the immobilized cells decreased despite culture in growth medium (DK medium). Caffeine production appeared to have a negative correlation with the growth of the immobilized cells throughout the subcultures.
ISSN:0721-7714
1432-203X
DOI:10.1007/bf00232086