Safflower microsomes catalyse oil accumulation in vitro: A model system

Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol from sn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activ...

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Bibliographic Details
Published in:Planta 1986-03, Vol.169 (1), p.33-37
Main Authors: Stobart, A.K. (Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Food Hygiene), Stymne, S, Hoeglund, S
Format: Article
Language:English
Subjects:
ACEITES VEGETALES
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biosynthesis
Boundary layers
Carthamus
CARTHAMUS TINCTORIUS
Cell membranes
Cotyledons
CYTOPLASMIC ORGANELLES
Fatty acids
Fundamental and applied biological sciences. Psychology
Glycerol
HUILE VEGETALE
Inhaltsstoff
Lipid bodies
Lipids
METABOLISM
METABOLISME
METABOLISMO
Microsomes
Mikrosom
Oelkoerper
ORGANITE CELLULAIRE
ORGANULOS CITOPLASMATICOS
Other biological molecules
Phosphatidylcholines
PLANT OILS
Samen
Stoffwechsel
Triglycerides
Zellmerkmal
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Summary:Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol from sn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activities which were compatible with oil accumulation observed in vivo. Reaction mixtures which contained the microsomes took on a white soup-like appearance as triacylglycerol synthesis proceeded and sufficient oil was produced to form a white fat-pad at the surface after centrifugation. The development of the oil bodies in the microsomal membranes was studied by electron microscopy and showed that lipid droplets were formed in or on the membrane surface and were then released as apparently naked entities into the surrounding medium. The ontogeny of the oil droplet in vitro is discussed in terms of oil-body formation in vivo.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF01369772