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Safflower microsomes catalyse oil accumulation in vitro: A model system
Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol from sn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activ...
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| Published in: | Planta 1986-03, Vol.169 (1), p.33-37 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c270t-2c26636aefb1c0dc1ff8da12603a1a583a9d7fba7b6e92a295751dc18d3a03ca3 |
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| cites | cdi_FETCH-LOGICAL-c270t-2c26636aefb1c0dc1ff8da12603a1a583a9d7fba7b6e92a295751dc18d3a03ca3 |
| container_end_page | 37 |
| container_issue | 1 |
| container_start_page | 33 |
| container_title | Planta |
| container_volume | 169 |
| creator | Stobart, A.K. (Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Food Hygiene) Stymne, S Hoeglund, S |
| description | Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol from sn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activities which were compatible with oil accumulation observed in vivo. Reaction mixtures which contained the microsomes took on a white soup-like appearance as triacylglycerol synthesis proceeded and sufficient oil was produced to form a white fat-pad at the surface after centrifugation. The development of the oil bodies in the microsomal membranes was studied by electron microscopy and showed that lipid droplets were formed in or on the membrane surface and were then released as apparently naked entities into the surrounding medium. The ontogeny of the oil droplet in vitro is discussed in terms of oil-body formation in vivo. |
| doi_str_mv | 10.1007/BF01369772 |
| format | article |
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The development of the oil bodies in the microsomal membranes was studied by electron microscopy and showed that lipid droplets were formed in or on the membrane surface and were then released as apparently naked entities into the surrounding medium. The ontogeny of the oil droplet in vitro is discussed in terms of oil-body formation in vivo.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/BF01369772</identifier><identifier>PMID: 24232426</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>ACEITES VEGETALES ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Biosynthesis ; Boundary layers ; Carthamus ; CARTHAMUS TINCTORIUS ; Cell membranes ; Cotyledons ; CYTOPLASMIC ORGANELLES ; Fatty acids ; Fundamental and applied biological sciences. Psychology ; Glycerol ; HUILE VEGETALE ; Inhaltsstoff ; Lipid bodies ; Lipids ; METABOLISM ; METABOLISME ; METABOLISMO ; Microsomes ; Mikrosom ; Oelkoerper ; ORGANITE CELLULAIRE ; ORGANULOS CITOPLASMATICOS ; Other biological molecules ; Phosphatidylcholines ; PLANT OILS ; Samen ; Stoffwechsel ; Triglycerides ; Zellmerkmal</subject><ispartof>Planta, 1986-03, Vol.169 (1), p.33-37</ispartof><rights>Springer-Verlag Berlin Heidelberg 1987</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c270t-2c26636aefb1c0dc1ff8da12603a1a583a9d7fba7b6e92a295751dc18d3a03ca3</citedby><cites>FETCH-LOGICAL-c270t-2c26636aefb1c0dc1ff8da12603a1a583a9d7fba7b6e92a295751dc18d3a03ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23378066$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23378066$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,58237,58470</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8259530$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24232426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stobart, A.K. (Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Food Hygiene)</creatorcontrib><creatorcontrib>Stymne, S</creatorcontrib><creatorcontrib>Hoeglund, S</creatorcontrib><title>Safflower microsomes catalyse oil accumulation in vitro: A model system</title><title>Planta</title><addtitle>Planta</addtitle><description>Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol from sn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activities which were compatible with oil accumulation observed in vivo. Reaction mixtures which contained the microsomes took on a white soup-like appearance as triacylglycerol synthesis proceeded and sufficient oil was produced to form a white fat-pad at the surface after centrifugation. The development of the oil bodies in the microsomal membranes was studied by electron microscopy and showed that lipid droplets were formed in or on the membrane surface and were then released as apparently naked entities into the surrounding medium. The ontogeny of the oil droplet in vitro is discussed in terms of oil-body formation in vivo.</description><subject>ACEITES VEGETALES</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Boundary layers</subject><subject>Carthamus</subject><subject>CARTHAMUS TINCTORIUS</subject><subject>Cell membranes</subject><subject>Cotyledons</subject><subject>CYTOPLASMIC ORGANELLES</subject><subject>Fatty acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerol</subject><subject>HUILE VEGETALE</subject><subject>Inhaltsstoff</subject><subject>Lipid bodies</subject><subject>Lipids</subject><subject>METABOLISM</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>Microsomes</subject><subject>Mikrosom</subject><subject>Oelkoerper</subject><subject>ORGANITE CELLULAIRE</subject><subject>ORGANULOS CITOPLASMATICOS</subject><subject>Other biological molecules</subject><subject>Phosphatidylcholines</subject><subject>PLANT OILS</subject><subject>Samen</subject><subject>Stoffwechsel</subject><subject>Triglycerides</subject><subject>Zellmerkmal</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNpF0MtrGzEQBnBRWhLncemx0KJDDiHgZCStXrmlebiFQA5pz8tYK5UNWiuVdlv830fGTnIQI5gfH8xHyGcG5wxAX3y_AyaU1Zp_IDPWCD7n0JiPZAZQ_2CF3CcHpTwB1KXWe2SfN1zUp2Zk8YghxPTfZzr0LqeSBl-owxHjunia-kjRuWmYIo59WtF-Rf_1Y06X9IoOqfORlnUZ_XBEPgWMxR_v5iH5fXf76_rH_P5h8fP66n7uuIZxzh1XSij0YckcdI6FYDpkXIFAhtIItJ0OS9RL5S1HbqWWrDLTCQThUByS023uc05_J1_GduiL8zHiyqeptKyRlklpGlvp2ZZuzirZh_Y59wPmdcug3RTXvhdX8bdd7rQcfPdGX5uq4GQHsDiMIePK9eXNGS6tFFDZ1y17KmPK7zFCaANqE_Nluw-YWvyTa8TNrVELMFaLFwUxhkA</recordid><startdate>198603</startdate><enddate>198603</enddate><creator>Stobart, A.K. 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Dept. of Food Hygiene) ; Stymne, S ; Hoeglund, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-2c26636aefb1c0dc1ff8da12603a1a583a9d7fba7b6e92a295751dc18d3a03ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>ACEITES VEGETALES</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Boundary layers</topic><topic>Carthamus</topic><topic>CARTHAMUS TINCTORIUS</topic><topic>Cell membranes</topic><topic>Cotyledons</topic><topic>CYTOPLASMIC ORGANELLES</topic><topic>Fatty acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycerol</topic><topic>HUILE VEGETALE</topic><topic>Inhaltsstoff</topic><topic>Lipid bodies</topic><topic>Lipids</topic><topic>METABOLISM</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>Microsomes</topic><topic>Mikrosom</topic><topic>Oelkoerper</topic><topic>ORGANITE CELLULAIRE</topic><topic>ORGANULOS CITOPLASMATICOS</topic><topic>Other biological molecules</topic><topic>Phosphatidylcholines</topic><topic>PLANT OILS</topic><topic>Samen</topic><topic>Stoffwechsel</topic><topic>Triglycerides</topic><topic>Zellmerkmal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stobart, A.K. (Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Food Hygiene)</creatorcontrib><creatorcontrib>Stymne, S</creatorcontrib><creatorcontrib>Hoeglund, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stobart, A.K. (Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Food Hygiene)</au><au>Stymne, S</au><au>Hoeglund, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Safflower microsomes catalyse oil accumulation in vitro: A model system</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1986-03</date><risdate>1986</risdate><volume>169</volume><issue>1</issue><spage>33</spage><epage>37</epage><pages>33-37</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol from sn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activities which were compatible with oil accumulation observed in vivo. Reaction mixtures which contained the microsomes took on a white soup-like appearance as triacylglycerol synthesis proceeded and sufficient oil was produced to form a white fat-pad at the surface after centrifugation. The development of the oil bodies in the microsomal membranes was studied by electron microscopy and showed that lipid droplets were formed in or on the membrane surface and were then released as apparently naked entities into the surrounding medium. The ontogeny of the oil droplet in vitro is discussed in terms of oil-body formation in vivo.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>24232426</pmid><doi>10.1007/BF01369772</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1986-03, Vol.169 (1), p.33-37 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1459155849 |
| source | Springer LINK Archives; JSTOR Journals and Primary Sources |
| subjects | ACEITES VEGETALES Analytical, structural and metabolic biochemistry Biological and medical sciences Biosynthesis Boundary layers Carthamus CARTHAMUS TINCTORIUS Cell membranes Cotyledons CYTOPLASMIC ORGANELLES Fatty acids Fundamental and applied biological sciences. Psychology Glycerol HUILE VEGETALE Inhaltsstoff Lipid bodies Lipids METABOLISM METABOLISME METABOLISMO Microsomes Mikrosom Oelkoerper ORGANITE CELLULAIRE ORGANULOS CITOPLASMATICOS Other biological molecules Phosphatidylcholines PLANT OILS Samen Stoffwechsel Triglycerides Zellmerkmal |
| title | Safflower microsomes catalyse oil accumulation in vitro: A model system |
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