effect of presumptive polyadenylation signals on the expression of the CAT gene in transgenic tobacco

Promoter::gene fusions which differed only in the presumed polyadenylation signals attached to the 3' end of the gene, have been used to examine the effect of these signals on expression of the gene in protoplasts and in transgenic plants. The gene constructs were comprised of the 35S promoter,...

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Bibliographic Details
Published in:Plant cell reports 1989-04, Vol.8 (4), p.195-198
Main Authors: Hernandez, G, Cannon, F, Cannon, M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
chloramphenicol
chromosome mapping
DNA
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic technics
genetic techniques and protocols
genetic transformation
Methods. Procedures. Technologies
Miscellaneous
Molecular and cellular biology
Molecular genetics
Nicotiana tabacum
nucleotide sequences
Synthetic digonucleotides and genes. Sequencing
transferases
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Summary:Promoter::gene fusions which differed only in the presumed polyadenylation signals attached to the 3' end of the gene, have been used to examine the effect of these signals on expression of the gene in protoplasts and in transgenic plants. The gene constructs were comprised of the 35S promoter, the chloramphenicol acetyl transferase (CAT) gene and DNA sequences carrying the presumed polyadenylation signals of the nopaline synthase (nos) gene or of transcript 7 (T-7) from the T-DNA ofAgrobacterium. Our results show that levels of gene expression were not significantly affected by the orientation or absence of these sequences. We therefore suggest that the practice of cloning presumptive polyadenylation sequences at the 3' end of a gene for expression in plants may be unnecessary.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00778530