Partition Affinity Ligand Assay (PALA) : A simple binding assay procedure

In a competitive binding assay, the ligand to be quantified competes with a fixed amount of labeled ligand for the sites on a limiting amount of binding protein. The amount of label bound is therefore dependent on the ratio between native and labeled ligand. In a binding assay, one must separate the...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1982-01, Vol.7 (1-2), p.71-74
Main Authors: Ling, T G, Mattiasson, B
Format: Article
Language:English
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Summary:In a competitive binding assay, the ligand to be quantified competes with a fixed amount of labeled ligand for the sites on a limiting amount of binding protein. The amount of label bound is therefore dependent on the ratio between native and labeled ligand. In a binding assay, one must separate the free ligands from bound. The better the separation, the higher the sensitivity of the assay. But effective methods are often laborious and time-consuming and thus we have developed a novel approach, the Partition Affinity Ligand Assay (PALA).
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02798626