Mapping of ripening-related or -specific cDNA clones of tomato (Lycopersicon esculentum)

Nineteen ripening-related or -specific clones from Lycopersicon esculentum were mapped via RFLP analysis using an F2 population from the cross L. esculentum x L. pennellii and cDNA or genomic clones of known map location. The map produced using cDNA and genomic clones of known map location correspon...

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Bibliographic Details
Published in:Theoretical and applied genetics 1990-04, Vol.79 (4), p.489-496
Main Authors: Kinzer, S.M, Schwager, S.J, Mutschler, M.A
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
chromosome mapping
Classical and quantitative genetics
Classical and quantitative genetics. Population genetics. Molecular genetics
Classical genetics, quantitative genetics, hybrids
clones
crossing
DNA
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
genetic polymorphism
Genetics and breeding of economic plants
Genetics of eukaryotes. Biological and molecular evolution
genomics
loci
mutants
polygalacturonase
Pteridophyta, spermatophyta
restriction fragment length polymorphism
ripening
Solanum
Solanum lycopersicum var. lycopersicum
Solanum pennellii var. pennellii
Vegetals
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Summary:Nineteen ripening-related or -specific clones from Lycopersicon esculentum were mapped via RFLP analysis using an F2 population from the cross L. esculentum x L. pennellii and cDNA or genomic clones of known map location. The map produced using cDNA and genomic clones of known map location corresponded well with previously published maps of tomato. The number of loci detected for each ripening-related or-specific clone varied from one to seven. These loci were located on all 12 chromosomes of the tomato genome. There was no significant clustering of ripening-related or-specific genes. Regions of very low recombination were observed. The clone for polygalacturonase (TOM6) mapped to a single region on chromosome 10, the same chromosome as the nor and alc ripening mutants. To fine map this chromosome, two backcross populations were produced from the cross of L. esculentum x L. pimpenillifolium, in which the esculentum parents used were homozygous for either the alc or the nor. The coding region for polygalacturonase is functionally unlinked to either of these two ripening mutants.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00226158