Alkaloid production by transformed root cultures of Catharanthus roseus

Transformed roots of Catharanthus roseus were obtained following infection of detached leaves with Agrobacterium rhizogenes. Roots would not grow in full strength Gamborg's B5 medium but would grow satisfactorily if the medium was diluted to one half strength. Little alkaloid appeared in the gr...

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Bibliographic Details
Published in:Plant cell reports 1988-08, Vol.7 (5), p.309-312
Main Authors: Parr, A.J, Peerless, A.C.J, Hamill, J.D, Waltaon, N.J, Robins, R.J, Rhodes, M.J.C
Format: Article
Language:English
Subjects:
agarobacterium rhizogenes
Agrobacterium
alkaloids
biosynthesis
Catharanthus roseus
culture media
genetic transformation
growth rate
roots
tissue culture
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Summary:Transformed roots of Catharanthus roseus were obtained following infection of detached leaves with Agrobacterium rhizogenes. Roots would not grow in full strength Gamborg's B5 medium but would grow satisfactorily if the medium was diluted to one half strength. Little alkaloid appeared in the growth medium but root tissue contained a high level and wide variety of alkaloids. Ajmalicine, serpentine, vindolinine and catharanthine were prominent components. Vinblastine could also be detected by a combination of HPLC and radioimmunoassay, though at a level of only 0.05μg/g dry weight.
ISSN:0721-7714
1432-203X
DOI:10.1007/bf00269925