Developmental and hormonal regulation of beta-1,3-glucanase in tobacco

A highly sensitive and specific "rocket" immunoassay was used to measure the content of an endo-type β-1,3-glucanase (EC 3.2.1.39) in tissues of Nicotiana tabacum L. cv. Havana 425. We show that the accumulation of β-1,3-glucanase in cultured pith-parenchyma tissue is blocked by combinatio...

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Bibliographic Details
Published in:Planta 1986-02, Vol.167 (2), p.206-211
Main Authors: Felix, G. (Friedrich Miescher Inst., Basel (Switzerland)), Meins, F. Jr
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Agronomy. Soil science and plant productions
Auxin
AUXINAS
AUXINE
AUXINS
Biological and medical sciences
CITOCININAS
Cytokinin
CYTOKININE
CYTOKININS
Economic plant physiology
Entwicklung
Enzymaktivitaet
Enzymes
ENZYMIC ACTIVITY
Fundamental and applied biological sciences. Psychology
Gewebe
GLICOSIDASAS
GLYCOSIDASE
GLYCOSIDASES
Hormonal regulation
Hormones
Leaves
Metabolism
Nicotiana
NICOTIANA TABACUM
Nutrition. Photosynthesis. Respiration. Metabolism
Physiological regulation
Plant growth regulators
Plant physiology and development
Plant roots
Plants
Regulation
Wachstumsregulator
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Summary:A highly sensitive and specific "rocket" immunoassay was used to measure the content of an endo-type β-1,3-glucanase (EC 3.2.1.39) in tissues of Nicotiana tabacum L. cv. Havana 425. We show that the accumulation of β-1,3-glucanase in cultured pith-parenchyma tissue is blocked by combinations of the auxin, α-naphthaleneacetic acid (NAA), and the cytokinin, kinetin. When tissues pre-incubated for 7 d on complete medium containing 2.0 mg·l-1 NAA and 0.3 mg·l-1 kinetin are transferred onto medium without hormones or with either hormone added separately, the β-1,3-glucanase content expressed per mg soluble protein increases approx. ten fold over a 7-d period. Under these inductive conditions, up to approx. 5% of the soluble protein is β-1,3-glucanase. The induction is inhibited by >90% when tissues are cultured over the same period on medium containing both hormones. This β-1,3-glucanase is developmentally regulated in the intact plant. It is a major component of the soluble protein in the lower leaves and roots but is not detectable in leaves near the top of the plant.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00391416