Production of aposporous gametophytes from Drymoglossum piloselloides (L.) Presl. frond strips

Strips of Drymoglossum piloselloides fronds in a modified Murashige and Skoog medium in 1% agar produced aposporous gametophytes in about 2 months. Young fronds showed a greater ability to develop aposporous gametophytes than older fronds. The addition of kinetin to the medium improved the ability o...

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Bibliographic Details
Published in:Plant cell reports 1988-03, Vol.7 (2), p.142-143
Main Authors: Kwa, S.H, Wee, Y.C, Loh, C.S
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
culture media
culture techniques
Economic plant physiology
Eukaryotic cell cultures
ferns and fern allies
Fundamental and applied biological sciences. Psychology
gametogenesis
In vitro culture
In vitro propagation: entire plant regeneration from tissues and cell cultures
kinetin
Methods. Procedures. Technologies
naphthaleneacetic acid
Plant cells and fungal cells
plant development
Plant physiology and development
Polypodiopsida
tissue culture
Tissue cultures, protoplasts
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Summary:Strips of Drymoglossum piloselloides fronds in a modified Murashige and Skoog medium in 1% agar produced aposporous gametophytes in about 2 months. Young fronds showed a greater ability to develop aposporous gametophytes than older fronds. The addition of kinetin to the medium improved the ability of older fronds while the presence of 1-naphthaleneacetic acid enhanced the effect of kinetin.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00270125