Monoclonal antibodies to a higher-plant nitrate reductase: differential inhibition of enzyme activities

A set of monoclonal antibodies has been raised against NADH-nitrate reductase (NR; EC 1.6.6.1) from spinach (Spinacea oleracea L.) leaves. Antibodies were screened by enzyme-linked immunosorbent assay and by their ability to inhibit various activities of the enzyme. The six monoclonals selected (AFR...

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Bibliographic Details
Published in:Planta 1985-01, Vol.165 (1), p.114-119
Main Authors: Notton, B.A, Fido, R.J, Galfre, G
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Analytical, structural and metabolic biochemistry
Antibodies
Antiserum
Barley
Biological and medical sciences
Bone marrow
Economic plant physiology
enzyme activity
Enzyme linked immunosorbent assay
Enzymes
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Molybdenum
Monoclonal antibodies
nitrate reductase
Nitrates
Nutrition. Photosynthesis. Respiration. Metabolism
Oxidoreductases
Spinacea oleracea
Spinach
Spinacia oleracea
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Summary:A set of monoclonal antibodies has been raised against NADH-nitrate reductase (NR; EC 1.6.6.1) from spinach (Spinacea oleracea L.) leaves. Antibodies were screened by enzyme-linked immunosorbent assay and by their ability to inhibit various activities of the enzyme. The six monoclonals selected (AFRC MAC 74 to 79) are all gamma globulins; four (MAC 74 to 77) inhibit all terminal donating activities (NADH-NR; flavin mononucleotide, reduced form (FMNH2)-NR; and methyl viologen, reduced form (MV)-NR) and two (MAC 78 and 79) inhibit the acceptor activities (NADH-NR, and NADH-cytochrome c reductase). MAC 74 to 77 inhibit the NADH-NR activity of crude extracts of a variety of species (mono- and dicotyledoneae) while MAC 78 and 79 are effective against spinach and marrow, but not oil-seed rape, cucumber, oats, wheat and barley.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00392219