Regeneration of plantlets from cell suspension culture derived callus of white poplar (Populus alba L.)

• Published in: Plant cell reports 1988-12, Vol.7 (7), p.567-570
• Main Authors: Park, Y.G, Son, S.H
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; callus; cell culture; cell suspension culture; culture media; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Plant cells and fungal cells; Populus alba; regeneration; shoots; stems
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/BF00272759

Friable calli derived from the stem tissues of Populus alba were used to establish cell suspension cultures which were characterized for in vitro growth and regeneration capacity. Suspended cells and callus recovered from these cells were maximal on a fresh weight basis using MS liquid medium containing 0.44 μM BAP and 4.52 μM 2,4-D. Shoot regeneration from the recovered callus was observed within 30 to 40 days of culture. The number of shoots was increased by subculturing the shoot-forming callus 2 to 3 times on MS medium supplemented with 19.7 μM 2iP and 0.05 μM IBA. Regenerated shoots were easily rooted on half-strength MS medium lacking growth regulators, and the plantlets were transferred to pots containing vermiculite for greenhouse growth.