Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes

'Hairy root' cultures of Beta vulgaris and Nicotiana rustica were established after roots were induced on plants following infection with Agrobacterium rhizogenes. The transformed cultures of B. vulgaris and N. rustica synthesised their characteristic secondary products, the betalain pigme...

Full description

Saved in:
Bibliographic Details
Published in:Plant cell reports 1986-04, Vol.5 (2), p.111-114
Main Authors: Hamill, J.D, Parr, A.J, Robins, R.J, Rhodes, M.J.C
Format: Article
Language:English
Subjects:
Agrobacterium
alkaloids
Beta vulgaris
Biological and medical sciences
Biotechnology
Cell cultures. Hybridization. Fusion
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
genetic transformation
in vitro
Methods. Procedures. Technologies
Miscellaneous
Molecular and cellular biology
Nicotiana rustica
Plant cells and fungal cells
roots
tissue culture
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:'Hairy root' cultures of Beta vulgaris and Nicotiana rustica were established after roots were induced on plants following infection with Agrobacterium rhizogenes. The transformed cultures of B. vulgaris and N. rustica synthesised their characteristic secondary products, the betalain pigments and nicotine alkaloids respectively, at levels comparable with those of in vivo roots from the same variety. Betalains were entirely retained inside the root tissue. In contrast, a proportion of the nicotine alkaloids was secreted into the medium. The potential of this type of 'in vitro' plant tissue culture for the production of valuable plant secondary products is identified and confirmed.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00269247