Analysis of microsomal flavoproteins from Phycomyces sporangiophores: Candidates for the blue-light photoreceptor

To help identify components of the blue-light photoreceptor system for phototropism in Phycomyces blakesleeanus Bgff., proteins from a microsomal fraction obtained from synchronous sporangiophores were studied. By two-dimensional gel electrophoresis, two proteins (FP1, FP2) with covalently bound fla...

Full description

Saved in:
Bibliographic Details
Published in:Planta 1985-04, Vol.163 (4), p.506-516
Main Authors: Pollock, J.A., Lipson, E.D., Sullivan, D.T.
Format: Article
Language:English
Subjects:
Adenosine triphosphatases
Azides
Cell membranes
Flavoproteins
Fluorescence
gel electrophoresis
Gels
Photoreceptors
Phototropism
Phycomyces
Phycomyces blakesleeanus
sporangiophores
Vanadates
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To help identify components of the blue-light photoreceptor system for phototropism in Phycomyces blakesleeanus Bgff., proteins from a microsomal fraction obtained from synchronous sporangiophores were studied. By two-dimensional gel electrophoresis, two proteins (FP1, FP2) with covalently bound flavins were found. FP1 has a molecular weight of 71000 and an isoelectric point of 6.6; FP2 has a molecular weight of 59000 and an isoelectric point of 6.5. These flavoproteins were purified by column chromatography and gel filtration while assaying for flavins by fluorescence. The relative concentrations of FP1 and FP2 were affected by light applied during growth. These flavoproteins are likely components of the blue-light photoreceptor complex mediating phototropism in Phycomyces.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00392708