Characteristics of Bupleurum falcatum plants propagated through somatic embryogenesis of callus cultures

• Published in: Plant cell reports 1986-10, Vol.5 (5), p.319-321
• Main Authors: Hiraoka, N, Kodama, T, Oyanagi, M, Nakano, S, Tomita, Y, Yamada, N, Iida, O, Satake, M
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; biosynthesis; Biotechnology; Bupleurum falcatum; Cell cultures. Hybridization. Fusion; Economic plant physiology; embryogenesis; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro culture; medicinal plants; Methods. Procedures. Technologies; micropropagation; Miscellaneous; Molecular and cellular biology; Plant cells and fungal cells; saponins; tissue culture
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/bf00268590

Various characteristics including the saponin content in the root of Bupleurum falcatum plants propagated in vitro through somatic embryogenesis of callus cultures were compared with those of the plants propagated by seeds. The asexually propagated plants had an aerial part of more uniform characteristics than those of sexually propagated ones. However, both the mean and variance of root weight of the former were significantly larger than those of the latter. As for the saponin content of the root on a dry weight basis, there was little difference between the two groups. The amounts of saikosaponins c and d in a root were significantly larger in the asexually propagated plants than in the sexually propagated ones.