ATP-induced calcium mobilization and inositol 1,4,5-trisphosphate formation in H-35 hepatoma cells

• Published in: FEBS letters 1986-08, Vol.204 (2), p.189-192
• Main Authors: Horstman, Debra A., Tennes, Karin A., Putney, James W.
• Format: Article
• Language: English
• Subjects: (H-35 cell) ATP receptor Ca2+-mobilizing receptor Purinergic receptor Inositol trisphosphate; (H-35 cell)ATP receptorCa 2+-mobilizing receptor Purinergic receptorInositol trisphosphate; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Lipids; Other biological molecules
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)80809-2

Addition of ATP (but not epinephrine, angiotensin II, vasopressin, or platelet-activating factor) to H-35 hepatoma cells whose cellular lipids have been pre-labelled with [ 3H]inositol, causes a rapid increase in [ 3H]inositol trisphosphate. In H-35 cells pre-incubated in the presence of 45Ca 2+, ATP causes a similarly rapid release of 45Ca 2+. The concentration-effect relationships for inositol trisphosphate formation and Ca 2+ efflux are similar to those reported previously for differentiated hepatocytes. These results demonstrate that at least one of the Ca 2+-mobilizing receptors normally found on hepatocytes is functionally retained in the H-35 hepatoma cell line and thus could provide a useful model for the study of these receptor mechanisms in liver.