Evaluation of circulating Transforming growth factor-beta1, Glypican-3 and Golgi protein-73 mRNAs expression as predictive markers for hepatocellular carcinoma in Egyptian patients

Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor - beta1...

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Bibliographic Details
Published in:Molecular biology reports 2013-12, Vol.40 (12), p.7069-7075
Main Authors: Ibrahim, Gehan H., Mahmoud, Moushira A., Aly, Nagwa M.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Biomedical and Life Sciences
Carcinoma, Hepatocellular - blood
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - pathology
Demography
Egypt
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genetic markers
Glypicans - blood
Glypicans - genetics
Hepatitis C, Chronic - blood
Hepatitis C, Chronic - genetics
Histology
Humans
Life Sciences
Liver cancer
Liver Neoplasms - blood
Liver Neoplasms - genetics
Liver Neoplasms - pathology
Male
Membrane Proteins - blood
Membrane Proteins - genetics
Middle Aged
Molecular biology
Morphology
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA, Messenger - blood
RNA, Messenger - genetics
Transforming Growth Factor beta1 - blood
Transforming Growth Factor beta1 - genetics
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Summary:Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor - beta1 ( TGF - β1 ), Glypican - 3 ( GPC3 ), and Golgi protein - 73 ( GP73 ) mRNAs as early biomarkers in HCC Egyptian patients chronically infected with hepatitis C virus (HCV) in comparison with serum alpha-fetoprotein (AFP). Using semi-quantitative RT-PCR and densitometry analysis, circulating TGF - β1 , GPC3 , and GP73 mRNAs expressions were estimated in 15 healthy adults, 15 chronic HCV (CHC) patients and 25 HCC patients. Serum GP73 expression percentage in HCC group was significantly higher than controls (100 vs. 40 %, P  ≤ 0.001) and when compared to elevated serum AFP levels (100 vs. 36 %, P  ≤ 0.001). TGF - β1 and GP73 expression means were also higher in HCC patients than controls and CHC patients ( P  
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-2829-3