UBE2Q1 expression in human colorectal tumors and cell lines

Colorectal cancer is the third most common cancer in the world. Ubiquitin–proteasome system has shown to be activated in colorectal and other malignancies. UBE2Q1 is a novel human gene that encodes a putative E2 ubiquitin conjugating enzyme. Here, we investigated the expression pattern of UBE2Q1 gen...

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Bibliographic Details
Published in:Molecular biology reports 2013-12, Vol.40 (12), p.7045-7051
Main Authors: Shafiee, Sayed Mohammad, Seghatoleslam, Atefeh, Nikseresht, Mohsen, Hosseini, Seyed Vahid, Alizadeh-Naeeni, Mahvash, Safaei, Akbar, Owji, Ali Akbar
Format: Article
Language:English
Subjects:
Adult
Aged
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Cell Line, Tumor
Colorectal cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Female
Gene Expression Regulation, Neoplastic
Histology
Humans
Life Sciences
Male
Middle Aged
Molecular biology
Morphology
Proteins
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transfection
Tumors
Ubiquitin-Conjugating Enzymes - genetics
Ubiquitin-Conjugating Enzymes - metabolism
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Summary:Colorectal cancer is the third most common cancer in the world. Ubiquitin–proteasome system has shown to be activated in colorectal and other malignancies. UBE2Q1 is a novel human gene that encodes a putative E2 ubiquitin conjugating enzyme. Here, we investigated the expression pattern of UBE2Q1 gene in cell lines and tissues from human colorectal tumors. Quantitative (q) RT-PCR were employed to evaluate the expression levels of the mRNA for UBE2Q1 in colorectal cancer cell lines (HT29/219, LS180, SW742, Caco2, HTC116, SW48, SW480 and SW1116). Expression of UBE2Q1 at the protein levels were assessed by Western blotting in cell lines as well as in 43 human colorectal tumor tissues. All cell lines tested expressed UBE2Q1 gene at the level of both mRNA and protein, with the SW1116 line representing the lowest level of expression. The cell lines HT29/219 and SW742 showed the highest levels of UBE2Q1 protein and mRNA respectively. When compared to corresponding normal tissues, malignant parts of colorectal tumors showed increased levels of UBE2Q1 immunoreactivity in 32 (74.42 %) of cases. These data suggest that UBE2Q1 is differentially expressed in colorectal cell lines and shows overexpression in colorectal tumors.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-2824-8