Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus notopterus (Pallas, 1769)

Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence with...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports 2013-12, Vol.40 (12), p.6625-6631
Main Authors: Gupta, Arti, Lal, Kuldeep K., Punia, Peyush, Singh, Rajeev K., Mohindra, Vindhya, Sah, Rama S., Kumar, Rajesh, Luhariya, Rupesh K., Dwivedi, Arvind K., Masih, Prachi, Mishra, R. M., Jena, J. K.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Biomedical and Life Sciences
Fishes - genetics
Genetic diversity
Genetic Loci - genetics
Genetic Markers
Histology
Life Sciences
Microsatellite Repeats - genetics
Molecular biology
Morphology
Polymorphism
Polymorphism, Genetic
Rivers
Satellite DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus . Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity ( P  
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-2776-z