Molecular detection of Ehrlichia canis in dogs from the Pantanal of Mato Grosso State, Brazil

The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were...

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Published in:Revista brasileira de parasitologia veterinaria 2013-01, Vol.22 (1), p.114-118
Main Authors: Santos, Luana Gabriela Ferreira Dos, Melo, Andréia Lima Tomé, Moraes-Filho, Jonas, Witter, Rute, Labruna, Marcelo Bahia, Aguiar, Daniel Moura de
Format: Article
Language:English
Subjects:
Animals
Brazil
DNA, Bacterial - blood
Dog Diseases - blood
Dog Diseases - diagnosis
Dogs
Ehrlichia canis - genetics
Ehrlichiosis - blood
Ehrlichiosis - diagnosis
Ehrlichiosis - veterinary
Molecular Diagnostic Techniques
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Summary:The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region.
ISSN:1984-2961
DOI:10.1590/S1984-29612013005000013