A novel approach for assessing cardiac fibrosis using label-free second harmonic generation

To determine whether second harmonic generation (SHG) can be used as a novel and improved label-free technique for detection of collagen deposition in the heart. To verify whether SHG will allow accurate quantification of altered collagen deposition in diseased hearts following hypertrophic remodell...

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Bibliographic Details
Published in:The International Journal of Cardiovascular Imaging 2013-12, Vol.29 (8), p.1733-1740
Main Authors: Martin, Tamara P., Norris, Greg, McConnell, Gail, Currie, Susan
Format: Article
Language:English
Subjects:
Animals
Azo Compounds
Cardiac Imaging
Cardiology
Cardiomegaly - diagnosis
Cardiomegaly - diagnostic imaging
Cardiomegaly - metabolism
Cardiomegaly - pathology
Cell Proliferation
Cells, Cultured
Collagen - metabolism
Coloring Agents
Disease Models, Animal
Fibroblasts - metabolism
Fibroblasts - pathology
Fibrosis
Image Interpretation, Computer-Assisted
Imaging
Male
Medicine
Medicine & Public Health
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton
Myocardium - metabolism
Myocardium - pathology
Original Paper
Radiology
Staining and Labeling - methods
Ultrasonography
Ventricular Remodeling
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Summary:To determine whether second harmonic generation (SHG) can be used as a novel and improved label-free technique for detection of collagen deposition in the heart. To verify whether SHG will allow accurate quantification of altered collagen deposition in diseased hearts following hypertrophic remodelling. Minimally invasive transverse aortic banding (MTAB) of mouse hearts was used to generate a reproducible model of cardiac hypertrophy. Physiological and functional assessment of hypertrophic development was performed using echocardiography and post-mortem analysis of remodelled hearts. Cardiac fibroblasts were isolated from sham-operated and hypertrophied hearts and proliferation rates compared. Multi-photon laser scanning microscopy was used to capture both two-photon excited autofluorescence (TPEF) and SHG images simultaneously in two channels. TPEF images were subtracted from SHG images and the resulting signal intensities from ventricular tissue sections were calculated. Traditional picrosirius red staining was used to verify the suitability of the SHG application. MTAB surgery induced significant hypertrophic remodelling and increased cardiac fibroblast proliferation. A significant increase in the density of collagen fibres between hypertrophic and control tissues ( p  
ISSN:1569-5794
1573-0743
1875-8312
DOI:10.1007/s10554-013-0270-2